西井 亘

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特許技術者


東京大学で農芸化学、同大学院で生物化学を専攻。東京薬科大学助手、理化学研究所研究員を経て、現在、理化学研究所客員主幹研究員。タンパク質科学、創薬科学を中心とした、ライフサイエンス研究に従事。2018年4月本事務所に入所。

Expertise

生化学、分子生物学、構造生物学、タンパク質科学、創薬科学、有機化学、微生物学

Education

  • 東京大学農学部農芸化学科卒業
  • 東京大学大学院理学系研究科生物化学専攻単位取得退学
  • 理学博士(東京大学)

Publications

  • Nishii, W., Niino-Kukimoto, M, Terada, T., Shirouzu, M. Muramatsu, T., Kojima, M, Kihara, H. & Yokoyama, S., "A redox switch shapes the Lon protease exit pore to facultatively regulate proteolysis". Nature Chemical Biology, 11, 46-51 (2015).
  • Nishii, W., Kubota, K. & Takahashi, K., 
"The P1 and P1' specificities of Physarolisin I, a serine-carboxyl peptidase from true slime mold Physarum polycephalum". 
Bioscience Biotechnology, and Biochemistry 73, 1168-71 (2009).
  • Nishii, W., Suzuki, T., Nakada, M., Kim, Y.-T., Muramatsu, T. & Takahashi, K.,
 "Cleavage mechanism of ATP-dependent Lon protease toward ribosomal S2 protein",
 FEBS Letters 579, 6846-6850 (2005).
  • Nishii, W. & Takahashi, K., "Determination of the cleavage sites in SulA, a cell division inhibitor, by ATP-dependent HslVU protease from Escherichia coli", 
FEBS Letters 553, 351-354 (2003).
  • Nishii, W., Kuriyama, H. & Takahashi, K., "The Physarum polycephalum php gene encodes a unique cold-adapted serine-carboxyl protease, physarolisin II", 
FEBS Letters 546, 340-344 (2003).
  • Nishii, W., Ueki, T., Miyashita, R., Kojima, M., Kim, Y.-T., Sasaki, N., Murakami-Murofushi, K. & Takahashi, K.
, "Structural and enzymatic characterization of physarolisin (formerly physaropepsin) proves that it is a unique serine-carboxyl proteinase", 
Biochemical and Biophysical Research Communications 301, 1023-1029 (2003).
  • Nishii, W., Shoda, T., Matsumoto, N., Nakamura, T., Kudo, Y. & Takahashi, K., "In situ visualization of caspase-1-like activity associated with promotion of hippocampal cell death”
FEBS Letters 518, 149-53 (2002).
  • Nishii, W., Maruyama, T., Matsuoka, R., Muramatsu, T. & Takahashi K.
“The unique sites in SulA protein preferentially cleaved by ATP-dependent Lon protease from Escherichia coli”
European Journal of Biochemistry 269, 451-7 (2002).
  • Nishii, W., Muramatsu, T., Kuchino, Y., Yokoyama, S. & Takahashi K., 
"Partial purification and characterization of a CAAX-motif-specific protease from bovine brain using a novel fluorometric assay". Journal of Biochemistry 122, 402-8 (1997).

Languages

  • 日本語
  • 英語

Affiliations/Certifications

  • 日本生化学会
  • 日本分子生物学会

Seminars