西井 亘 (博士)

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专利工程师


在东京大学学习了生物化学与生物物理之后、西井先生在东京药学与生命科学大学担任了助理教授、并在理化学研究所从事研究科学研究。他在生命科学研究方面拥有丰富的经验,专注于蛋白质科学和药物发现科学。

Expertise

生物化学,分子生物学,结构生物学,蛋白质科学,制药科学,药物发现科学,有机化学,微生物学

Education

  • 东京大学、农业学院农业化学系、学士
  • 东京大学、生物化学与生物物理系、科学研究生院、博士

Publications

  • Nishii, W., Niino-Kukimoto, M, Terada, T., Shirouzu, M. Muramatsu, T., Kojima, M, Kihara, H. & Yokoyama, S., "A redox switch shapes the Lon protease exit pore to facultatively regulate proteolysis". Nature Chemical Biology, 11, 46-51 (2015).
  • Nishii, W., Kubota, K. & Takahashi, K., 
"The P1 and P1' specificities of Physarolisin I, a serine-carboxyl peptidase from true slime mold Physarum polycephalum". 
Bioscience Biotechnology, and Biochemistry 73, 1168-71 (2009).
  • Nishii, W., Suzuki, T., Nakada, M., Kim, Y.-T., Muramatsu, T. & Takahashi, K.,
 "Cleavage mechanism of ATP-dependent Lon protease toward ribosomal S2 protein",
 FEBS Letters 579, 6846-6850 (2005).
  • Nishii, W. & Takahashi, K., "Determination of the cleavage sites in SulA, a cell division inhibitor, by ATP-dependent HslVU protease from Escherichia coli", 
FEBS Letters 553, 351-354 (2003).
  • Nishii, W., Kuriyama, H. & Takahashi, K., "The Physarum polycephalum php gene encodes a unique cold-adapted serine-carboxyl protease, physarolisin II", 
FEBS Letters 546, 340-344 (2003).
  • Nishii, W., Ueki, T., Miyashita, R., Kojima, M., Kim, Y.-T., Sasaki, N., Murakami-Murofushi, K. & Takahashi, K.
, "Structural and enzymatic characterization of physarolisin (formerly physaropepsin) proves that it is a unique serine-carboxyl proteinase", 
Biochemical and Biophysical Research Communications 301, 1023-1029 (2003).
  • Nishii, W., Shoda, T., Matsumoto, N., Nakamura, T., Kudo, Y. & Takahashi, K., "In situ visualization of caspase-1-like activity associated with promotion of hippocampal cell death”
FEBS Letters 518, 149-53 (2002).
  • Nishii, W., Maruyama, T., Matsuoka, R., Muramatsu, T. & Takahashi K.
“The unique sites in SulA protein preferentially cleaved by ATP-dependent Lon protease from Escherichia coli”
European Journal of Biochemistry 269, 451-7 (2002).
  • Nishii, W., Muramatsu, T., Kuchino, Y., Yokoyama, S. & Takahashi K., 
"Partial purification and characterization of a CAAX-motif-specific protease from bovine brain using a novel fluorometric assay". Journal of Biochemistry 122, 402-8 (1997).

Languages

  • 日语 (母语)
  • 英语

Affiliations/Certifications

  • The Japanese Biochemical Society
  • The Molecular Biology Society of Japan

Seminars